Mammalian Cell Culture is a highly sought-after lab technique that is often requested for graduate-level researcher positions in academia and industry. Our Mammalian Cell Culture course offers comprehensive laboratory-based tuition using a range of adherent & suspension cell lines and primary cells, thus providing a rare opportunity to gain hands on experience that is crucial for future careers in Biology, Biomedical Science, Pharmacology & Medical Research.

This course is designed to introduce students to the fundamental concepts and techniques of mammalian cell culture to help them carry out core cell culture techniques properly and safely. The course runs for 5 days of lab-based tuition and a total of 35 hours of delivery. Working in small groups, students receive comprehensive tuition with experienced scientists covering the laboratory requirements and legal regulations of cell culture in the UK.

The Mammalian Cell culture (5 Days) programme runs from 9:30am – 4pm for 5 consecutive days with an hour break for lunch. We teach at a very low student to tutor ratio (5:1) meaning that students are supervised and encouraged throughout their course.

The course content is ideal for undergraduate and postgraduate students who wish to freshen-up their skills and gain a better understanding of certain laboratory procedures. It is also ideal for applying for further research studies or scientific posts are recommended to attend this course or any more advanced courses that BioGrad Education offers.

We ask students to design their own experiments and allow students to make (safe) mistakes and learn from these. We believe this helps students to develop independent research skills and gain confidence in their own ability in the laboratory.

• All aspects of Good Cell Culture Practice including aseptic technique and biosafety.
• Sterile media preparation
• Passaging of suspension and adherent cell lines.
• Cryopreservation and resuscitation of cryopreserved cells.
• Cell counting & density calculations.
• Analysis of confluency, morphology and viability.
• Isolation of peripheral blood mononuclear cells (PBMCs) using density centrifugation.
• Cellular differentiation.
• Histological staining, brightfield & inverted microscopy.
• Cell viability assay (commonly used for in vitro drug predictions and nanotoxicology research).
• The laboratory results obtained by the students are analysed and discussed. Strong focus is set on the student acquiring a good aseptic technique through practical training and supervision.

Students attending this course are expected to have previous laboratory experience, therefore, a questionnaire must be completed prior to the commencement of your course to ensure you are able to meet the required competencies.

If you do not yet possess the necessary lab skills, you need to attend our 1 day ‘Introduction to laboratory skills’ course or the 5 days ‘Essential Laboratory Techniques’ course before commencing this course.

Attendees will benefit from having hands-on laboratory experience in a state-of-the-art industry laboratory at BioGrad Ltd, as well as from interacting closely with our senior scientists and fellow students throughout the course period.

Attendees will demonstrate an understanding of the concepts of mammalian cell culture and utilize lab protocols to carry out common mammalian cell culture techniques such as preparing sterile cell culture media, passaging of suspension and adherent cell lines, counting cells using the hemacytometer and the automated cell counter, freezing cells and resuspension of cell stocks, evaluating confluence and morphology of the cells, and isolation of white blood cells using density centrifugation techniques.

Attendees can also measure the cell viability, perform differentiation assay and histological staining technique as well as inverted microscopy.

Attendees can perform & analyse the results of a cell viability experiment as well as maintain detailed records of lab work.

Attendees will gain valuable insights into the range of careers and laboratory research opportunities available. We have links with many companies that recruit interns and graduates based on our recommendations. On completion of the course, your new skills will be endorsed by our lecturers on LinkedIn and a reference can be provided upon request.

Students attending any of our Level 4-6 and Level 7 courses must be confident and competent in a working laboratory. You must be able to walk in and start work selecting correct pipettes and tips, making molar solutions and serial dilutions to attend these courses. A questionnaire must be completed prior to commencement of your course to ensure you are able to meet these required competencies. If you do not yet possess the necessary skills, we recommend you attend one of our 1 day ‘Laboratory Skills’ courses prior to booking this course.

Our courses are under constant review to ensure they remain in-line with current protocols within the NHS, research, and industry. This means that course content may change.

"I would like to thank you for a well organized and insightful hands-on cell culture training. I have learnt a lot and feel more confident in speaking to customers and stake holders within the company. Keep up your good work!" - Nikolai K, Hamilton Company