The field of genetics and ‘gene cloning’ has been revolutionised by PCR, the Polymerase Chain Reaction. By clever design of primers this technique can be used for classical gene cloning, addition, mutation or deletion of genes, sequencing of DNA, and for the preparation of gene libraries for use in comparative gene expression studies or Next Generation Sequencing. The complete annotated genome sequences of many different organisms are available in free online databases (genomics), as are many analysis tools (bioinformatics).
This two day course offers laboratory-based tuition in the molecular biology techniques needed in almost any field of biological research. Students will be able to use their new skills to express a target gene in a cell line for drug discovery, produce recombinant proteins for therapeutic applications, or identifying species of micro-organisms for diagnosis of infectious diseases.
You will learn how to extract and purify nucleic acids using modern laboratory methods, then use extracted DNA in a polymerase chain reaction (PCR), and analyse the results by agarose gel electrophoresis. You will be introduced to free online programmes and databases that can be used to design primers for PCR, and practice using these online tools to identify species from a gene sequence.
This course introduces essential practical molecular biology skills and we expect that students will understand how to use basic laboratory equipment, make stock solutions, follow UK H&S policies including Risk Assessments and COSHH, and maintain a laboratory note book. Students without any previous laboratory experience should attend the laboratory skills course or laboratory skills summer school before commencing this course. The Discovering Practical Microbiology course and the Introduction to Mammalian Cell Culture form useful complements to this course.
Students are advised to wear comfortable clothes and shoes (ensuring legs and feet are covered when in the laboratory). We advise layered clothing as it can become warm during the course of the day, especially under a lab a coat and gloves.
All personal protective equipment is provided during the course however students should bring a note book for recording their results. Some students might find a laptop or tablet useful to work on the computer assignments and to download images of their gels to take away at the end of the course.
We will happily take photographs of students working in the laboratory which often serve as excellent profile pictures for work related social networking (such as LinkedIn). Our lectures will also endorse your new skills and provide a reference if requested.
We have active links with the recruitment agencies SRG and Network Scientific, students will be provided with the contact details of our recruitment consultants if requested, and references can be provided directly to these agencies.
Courses are carried out with high levels of staffing to encourage questions, interaction and discussion. Refreshments, lunch and snacks are provided during both days of the course; please advise BioGrad of specific dietary requirements at the time of booking.
Accommodation is not included with this course however we are happy to recommend local hotels at the time of booking.
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If you are booking this course more than 28 days in advance, you can pay a 25% deposit to secure your place. The outstanding balance is payable 28 days prior to the start of the course. You will be sent an invoice for the remaining balance 6 weeks before the course begins. You can also opt to pay for this course in full after registration.
If you are booking this course less than 28 days in advance, full payment is required after registration.
All payments are taken sucurely with PayPal. All major debit and credit cards are accepted and you don’t need to have a PayPal account.