Introduction to DNA Analysis & Bioinformatics
2 Day Course
£600
Available Dates
Introduction

The field of genetics and ‘gene cloning’ has been revolutionised by PCR, the Polymerase Chain Reaction. By clever design of primers this technique can be used for classical gene cloning, addition, mutation or deletion of genes, sequencing of DNA, and for the preparation of gene libraries for use in comparative gene expression studies or Next Generation Sequencing. The complete annotated genome sequences of many different organisms are available in free online databases (genomics), as are many analysis tools (bioinformatics).

Overview

This two-day course offers laboratory-based tuition in the molecular biology techniques needed in almost any field of biological research. Students will be able to use their new skills to express a target gene in a cell line for drug discovery, produce recombinant proteins for therapeutic applications, or identifying species of micro-organisms for diagnosis of infectious diseases.

You will learn how to extract and purify nucleic acids using modern laboratory methods, then use extracted DNA in a polymerase chain reaction (PCR) and analyse the results by agarose gel electrophoresis. You will be introduced to free online programmes and databases that can be used to design primers for PCR, and practice using these online tools to identify species from a gene sequence.

What to expect

The Introduction into DNA Analysis and Bioinformatics (2 Days) programme runs from 9:30am – 4pm for 2 consecutive days with an hour break for lunch. We teach at a very low student to tutor ratio (5:1) meaning that students are supervised and encouraged throughout their course.

Students are advised to wear comfortable clothes and shoes (ensuring legs and feet are covered when in the laboratory). We advise layered clothing as it can become warm during the course of the day, especially under a lab a coat and gloves.

All personal protective equipment is provided during the course however students should bring a notebook for recording their results. Some students might find a laptop or tablet useful to work on the computer assignments and to download images of their gels to take away at the end of the course.

Courses are carried out with high levels of staffing to encourage questions, interaction and discussion. Refreshments, lunch and snacks are provided during both days of the course; please advise BioGrad Education of specific dietary requirements at the time of booking.

Course Content

Day 1

  • Introduction to molecular biology techniques
  • Students collect own cheek cells for DNA extraction
  • Extraction of DNA from cheek cells
  • Introduction to the PCR method, and essential components of the reaction mixture
  • Students set up a PCR reaction mixture using their extracted DNA and programme the amplification cycle.
  • Introduction to online genomics and bioinformatics tools, and their use in primer design
  • Computer activity: design and check a set of PCR primers
  • Agarose gel electrophoresis of PCR samples
  • Discussion of results

Day 2

  • Introduction to RNA, its uses, and the pitfalls of working with it
  • Students collect own cheek cells for RNA extraction
  • Extraction of total RNA from cheek cells
  • Methods of messenger RNA extraction
  • Agarose gel electrophoresis of RNA samples
  • Discussion of results
  • Video on DNA sequencing and Next Generation Sequencing technologies
  • Computer activity: identification of species

This course introduces essential practical molecular biology skills and we expect that students on our level 7 courses will understand how to use basic laboratory equipment, make stock solutions, follow UK H&S policies including Risk Assessments and COSHH, and maintain a laboratory note book. Students without any previous laboratory experience should attend the laboratory skills course or laboratory skills summer school before commencing this course. The Discovering Practical Microbiology course and the Introduction to Mammalian Cell Culture form useful complements to this course.

We will happily take photographs of students working in the laboratory which often serve as excellent profile pictures for work related social networking (such as LinkedIn). Our lectures will also endorse your new skills and provide a reference if requested.

Prerequisites
  • This course is designed for post-graduates who wish to gain ‘real’ experience in setting up their own protocols.
  • This course introduces essential practical Geonomics and Bioinformatics however students attending any of our undergraduate and postgraduate courses must be confident and competent in a laboratory setting. Thus, a questionnaire must be completed prior to the commencement of your course to ensure you are able to meet the required competencies.
  • Students without any previous laboratory experience should attend our 1 day ‘Introduction to laboratory skills’ course or the 5 days ‘Essential Laboratory Techniques’ course before commencing this course
Learning Outcomes
  • Attendees will not only gain transferable laboratory skills but also valuable insights into the research opportunities and applications of these techniques.
  • Attendees will benefit from having hands-on laboratory experience in a state-of-the-art industry laboratory at BioGrad Education, as well as from interacting closely with our senior scientists and fellow students throughout the course period.
Course Endpoint

This course provides post-graduate scientists with transferable skills and techniques in the laboratory, to progress on into jobs or research projects which involve these skills on a day-to-day basis. These skills can be utilised as tools in postgraduate research projects, it may be techniques you may have not learnt before or want to refresh on before starting a new role or research project.

All of our courses provide attendees with valuable insights into the range of careers and laboratory research opportunities available. We have links with many companies that recruit interns and graduates based on our recommendations. On completion of the course, your new skills and laboratory techniques will be endorsed by our lecturers on LinkedIn and a reference can be provided upon request.

Course Details
Price

The cost of this course is £600

You can reserve your place on this course with a deposit of just £150.00 if you are booking more than 28 days in advance. The remaining balance is due four weeks prior to the course start date

You can also choose to pay for this course in 6 instalments if you are booking more than 26 weeks in advance, or 12 instalments if you are booking more than 54 weeks in advance

Location

Science Park, Liverpool, L3 5TF

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Duration

This course lasts for 2 days

9am - 5pm

There are up to four payment options available:
  • Pay in full at the time of booking
  • Pay in 2 - 25% deposit with the remainder due 28 days before the course commences (only available if booking more than 28 days in advance)
  • Pay in 6 - 6 equal, monthly payments (only available if booking more than 26 weeks in advance)
  • Pay in 12 - 12 equal, monthly payments (only available if booking more than 54 weeks in advance)
Available Course Dates
Current Academic Year 01/09/2023 to 01/09/2024
£600
23rd March - 24th March 2024
Location: Science Park, Liverpool
Only 6 places left on this course

Sorry, we don't have any of these courses planned at the moment in the next academic year.